Insertion junction: CLIP2.037734_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.037734
Chromosome:chromosome 4
Location:1959031
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g218850 RNP (1 of 1) K14846 - ribosome production factor 1 (RPF1); Putative small nucleolar ribonucleoprotein U3 protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCCTTCAGATTCCAGGACCCACTCACGTGCTGCAGGGGCTTCGGCGGC
Internal bar code:TTTGGAAGTCTTTACAATGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1191
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGCATTGGCAATGTTCAA
Suggested primer 2:CCGCCATGATCTCCTCATCC