| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.037736 |
| Chromosome: | chromosome 1 |
| Location: | 6579535 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g047100 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCGGCTACCCTTGGAAGTTTGGTGATGGCTTGAACCTGGATGCCGC |
| Internal bar code: | TGGATCCTGCTAAAGATTGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3414 |
| LEAP-Seq percent confirming: | 13.4615 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGCCAAACCGCTCATCA |
| Suggested primer 2: | CCTGTTCCAGTACGTGCTGT |