Insertion junction: CLIP2.037745_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.037745
Chromosome:chromosome 7
Location:4662800
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g344800 TNP21,CSB33 (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon); {"Probable transposon-derived protein of Chlamydomonas-Specific family B CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGCTGGCGCAAGCTGTGGGCGGTTTGGCTGGAGTCGTTCCAGGCCTGA
Internal bar code:ATAGATCAGTTTTTGTGCTATA

Confirmation - LEAP-Seq

LEAP-Seq distance:3769
LEAP-Seq percent confirming:47.619
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GCCAACTACATCACCACGGA
Suggested primer 2:TCGTTGCAGACTTTGACCGA