| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.037756 |
| Chromosome: | chromosome 12 |
| Location: | 7368320 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557650 | (1 of 100) IPR001810 - F-box domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGTACGGCGGCGGCTCCGCCGCCCCGCCTCCTGTCGGGCTGACGCCG |
| Internal bar code: | ACTCCCTGAATAGGTGATTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2195 |
| LEAP-Seq percent confirming: | 41.3043 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGACTGTGGTGGTGACC |
| Suggested primer 2: | TACAGGTCACGAGGGCTGTA |