| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.037787 |
| Chromosome: | chromosome 11 |
| Location: | 3520832 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477700 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATGACCAGTCACGTCAGTGGGGTGGACACGACTTTTCGCATTGCTAGC |
| Internal bar code: | GCGACTAGTCCCGATGAACAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1350 |
| LEAP-Seq percent confirming: | 98.2759 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGTTGCGGCAAGTCCTCT |
| Suggested primer 2: | AGAGCGCTGATGAGCCTTTT |