| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.037794 |
| Chromosome: | chromosome 3 |
| Location: | 2212159 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157350 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGCGGCAACGCCAGCGCGAGCAATGACTGGTGGACCGCGCCGCTGCTG |
| Internal bar code: | GCGGACGAGTGTGTCTATATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3147 |
| LEAP-Seq percent confirming: | 81.7204 |
| LEAP-Seq n confirming: | 76 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTCCCTCCTCCTAGGCAC |
| Suggested primer 2: | CTGCACAAACGACACCACAG |