| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.037801 |
| Chromosome: | chromosome 16 |
| Location: | 3723808 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682013 | (1 of 2) PTHR30545:SF2 - SUGAR FERMENTATION STIMULATION PROTEIN A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTACAAGAGGGACCTGCTGACGCAGTTCACGAGCCAGTGACGTGGAGA |
| Internal bar code: | GCGTGGAATGTCCCATTAAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2029 |
| LEAP-Seq percent confirming: | 98.2759 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACAGTAGCTGGGTTGCAG |
| Suggested primer 2: | ATGTTCCATCACGGTCACCC |