| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.037806 |
| Chromosome: | chromosome 2 |
| Location: | 1881232 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g087551 | (1 of 1) K17600 - vacuolar protein sorting-associated protein 54 (VPS54) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAGCGGCAGCTGCAACGGCAACGACGGCGGCGGCTGCCGCCGCCACG |
| Internal bar code: | TACGTCGAGCGATGGGTATCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3807 |
| LEAP-Seq percent confirming: | 53.8462 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCGCAGCATCCCTAGAG |
| Suggested primer 2: | GAGGTCGCCTACATCTTCGG |