| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.037808 |
| Chromosome: | chromosome 11 |
| Location: | 1998372 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467768 | (1 of 1) K10277 - lysine-specific demethylase 8 [EC:1.14.11.27] (KDM8, JMJD5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGAGTTTTGGTGCTTTGCTAAGGACGGACGGAGCTGGGGGGCTTGGGG |
| Internal bar code: | AAACATGTTTGCCGCGGGTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1137 |
| LEAP-Seq percent confirming: | 58.8235 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCCAGTCACGTCCTTGA |
| Suggested primer 2: | GCCACACTTTCATGCAAGCA |