| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.037810 |
| Chromosome: | chromosome 3 |
| Location: | 8339592 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199090 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAGGCACATGCACGTGCCCCCCGCCGGTGTCCCTGCCGGCCATCAGC |
| Internal bar code: | TGTGGGTTGTATCGATCCGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 447 |
| LEAP-Seq percent confirming: | 14.7059 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATAACCACGGTGGGTGTG |
| Suggested primer 2: | CACTATGACCTCTGCGCCAT |