| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.037815 |
| Chromosome: | chromosome 8 |
| Location: | 48964 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358200 | ACV4 | protein kinase; (1 of 6) PTHR23257//PTHR23257:SF529 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCCTGGCCCTGCAGCAGCGGCTGTGCGTCGTGGCCGGCGCCGTCGCC |
| Internal bar code: | GAAGGCACCGTGGTTGCTTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 438 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCACTGCGATCCTGAATC |
| Suggested primer 2: | GCAGGGCCTTACAACCTCAT |