Insertion junction: CLIP2.037821_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.037821
Chromosome:chromosome 3
Location:5349547
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g183200 CPC1 Central pair microtubule complex 1; (1 of 1) PTHR14919:SF0 - SPERM FLAGELLAR PROTEIN 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCGCGGCCTGACGCCGACATTGCCCACATAAATGCACAGGCACTTTGA
Internal bar code:TTGCTAGTTAAGGTCTTTTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3748
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:CAACAGCATCGTCGCATGAG
Suggested primer 2:CGCTAACCCAACCCCTCAAT