Insertion junction: CLIP2.037873_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.037873
Chromosome:chromosome 3
Location:699529
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145827 subtilisin-like Type II membrane endoprotease; (1 of 1) IPR000209//IPR003882//IPR015500 - Peptidase S8/S53 domain // Pistil-specific extensin-like protein // Peptidase S8, subtilisin-related CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCACTAAGTACGCGGATATCGGCGCGCCGGGTCGGCAGATCTACTCCAC
Internal bar code:AACGAGATAAGGTGTTAAGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:356
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GTTCGACGGTTTGGTGTGTG
Suggested primer 2:TTGCCCTGCAAATTCGTGTG