| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.037874 |
| Chromosome: | chromosome 17 |
| Location: | 3731086 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726250 | ATP12 | Assembly factor 2 for F1 mitochondrial ATP synthase; (1 of 1) K07556 - ATP synthase mitochondrial F1 complex assembly factor 2 (ATPeAF2, ATPAF2, ATP12) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCGCACCGCACCGGGGAGCCAGCGTCCCAGCGTTGCCACACGTTGGC |
| Internal bar code: | GGAGGTACTGAAGATTTTTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3778 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCTGCACTACTACTCGA |
| Suggested primer 2: | CATGAACAGGCGTCAGACCT |