Insertion junction: CLIP2.037879_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.037879
Chromosome:chromosome 12
Location:8528010
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g549250 (1 of 93) 6.3.2.19 - Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCAGCAACCTGCCGCTACCCAGCGCCTCGCTCACCACGGTGTGGCCCAT
Internal bar code:CTATTATAACATTTCGACTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1610
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CCCATGCACAGCTTTCGATG
Suggested primer 2:GGGTTCAAAACGTGGTCTGC