Insertion junction: CLIP2.037880_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.037880
Chromosome:chromosome 16
Location:6766272
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g672500 (1 of 1) PTHR28573//PTHR28573:SF1 - FAMILY NOT NAMED // SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGAGAGTACTTCCGCCGGACCTACGGGCACGCATCGACGCTCTTGTCT
Internal bar code:TCCCAGGCAATGTAAGACGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2979
LEAP-Seq percent confirming:5.66038
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:50
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:AAGATGTTGGAGCTCTGGGC
Suggested primer 2:CTTGGCTGCCCAACCATCTA