| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.037881 |
| Chromosome: | chromosome 10 |
| Location: | 244194 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGGGAAGCCACAAACGCACCCGCGCAAGTCTCGGCACCATCCCCGGC |
| Internal bar code: | AGATCCGTGGATCACCACCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2181 |
| LEAP-Seq percent confirming: | 57.1429 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGACGTGCACTTAGTCACT |
| Suggested primer 2: | CCTTTCCGCGCATGAAGATG |