| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.037886 |
| Chromosome: | chromosome 17 |
| Location: | 2754741 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g717850 | PHC8 | (1 of 1) IPR003882//IPR024616//IPR027417 - Pistil-specific extensin-like protein // Pherophorin // P-loop containing nucleoside triphosphate hydrolase; Pherophorin-chlamydomonas homolog 8 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAACAAAAGCGCTCGCATGCTCGCATGCATTGTTCTGTAGTCAAGAAA |
| Internal bar code: | ACCAATGGCATGTACGCACTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2621 |
| LEAP-Seq percent confirming: | 89.8551 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGTCTTCTGGGTGACAA |
| Suggested primer 2: | GTGATGCAAATCTCCGCACC |