Insertion junction: CLIP2.037895_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.037895
Chromosome:chromosome 3
Location:333264
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g144564 MMP13 Matrix metalloproteinase; (1 of 1) IPR008752//IPR024079 - Peptidase M11, gametolysin // Metallopeptidase, catalytic domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTGGCGGAGTTCAATGACCTGACGGACGTGCTGAGCCAGTTGGGGGTG
Internal bar code:TTATTGACATCTTCTCTATCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:267
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGATCTGAGACTCGCTGAGC
Suggested primer 2:GAACTTGCTGCTTGTGTGCA