| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.037911 |
| Chromosome: | chromosome 12 |
| Location: | 4577453 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521650 | (1 of 3) PTHR22753:SF1 - TRANSMEMBRANE PROTEIN 68 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGGGGGGGGAGGGAGGGCGAGGGGGCGAGGGGGAAGGGTTCAGGTGC |
| Internal bar code: | GTCAAATTTTTAGTATCGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 229 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGCCAAACTGGTGTCATG |
| Suggested primer 2: | TTCCGAACATTCTCAGCCCC |