Insertion junction: CLIP2.037944_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.037944
Chromosome:chromosome 1
Location:6753044
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g048200 (1 of 1) PTHR10887//PTHR10887:SF140 - DNA2/NAM7 HELICASE FAMILY // PROTEIN ERI-7 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCCCTGATACCAACCTGCTGTGCTTGACACGGTTGCGCACGTTGGGCA
Internal bar code:GACTAATCTTACACCCGCTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:3885
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:60
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:CTCATCCACCTTTCGGCTGT
Suggested primer 2:ATCGGATCCACATCGAACCG