| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.037946 |
| Chromosome: | chromosome 12 |
| Location: | 1862813 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512200 | MFT26 | Major facilitator superfamily transporter; (1 of 1) PTHR23504//PTHR23504:SF15 - FAMILY NOT NAMED // PROTEIN ZINC INDUCED FACILITATOR 1-RELATED | 3'UTR |
| Cre12.g512250 | RRP6b,RRP7 | 3'-5' endonuclease; (1 of 1) K12591 - exosome complex exonuclease RRP6 (RRP6, EXOSC10) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGCCCGTTGAGAGGGCCCGGCCGGCTCGCCATCTTAAGCGAGGACAG |
| Internal bar code: | CGGCCGAGTGATTCATCAGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1146 |
| LEAP-Seq percent confirming: | 65.3846 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCGGAACTGGTGTACTGT |
| Suggested primer 2: | CCAGTCAGGAATGGGACCAC |