Insertion junction: CLIP2.037953_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.037953
Chromosome:chromosome 10
Location:3065699
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g441100 PIGT,GPIT1,GPI16,GIT1,PIGT1 GPI transamidase component Gpi16; (1 of 1) K05292 - phosphatidylinositol glycan, class T (PIGT) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAGGGAAGGTTGGTTTACTCCCATCCCAAAGAAAAACCAAGACCCAGA
Internal bar code:TTACCAATGGACTTAAAACTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:988
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TGGAGTTAGCGCCTGTCAAG
Suggested primer 2:CATTAAAATCCCTGGCCGCG