Insertion junction: CLIP2.037960_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.037960
Chromosome:chromosome 6
Location:6915333
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g296850 FAP81,DLEC1 (1 of 8) IPR008962 - PapD-like; Flagellar Associated Protein 81 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCTGCGGGTGGTGCGCGTCCTCGTCCTTGGTCTGGCGGCGCGAAGCGC
Internal bar code:GGGTATGTAACATGAGGGGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:254
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TACGACGATTACATGGCGCA
Suggested primer 2:AGCCCCTTTACCTTGACGTG