Insertion junction: CLIP2.037963_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.037963
Chromosome:chromosome 5
Location:874492
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g246300 (1 of 1) PTHR13554//PTHR13554:SF10 - 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 5-RELATED // 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 5 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCCCTACCCCACCCCACCCCCTCATCACGCACTTGCACGCCGCGGCGC
Internal bar code:TCCGCTACAGGCCTCAACTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:541
LEAP-Seq percent confirming:89.1892
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTGGCTTTGCTTGGGAGG
Suggested primer 2:AAAAACTGTACCGCACACGC