| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.037967 |
| Chromosome: | chromosome 16 |
| Location: | 4599446 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671000 | NDA5 | Type-II NADH dehydrogenase; (1 of 1) K17872 - NADH:ubiquinone reductase (non-electrogenic) (ndh2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCCTGGGCACCTGGCGCAACCTCTCAACCCCTGCGGCGCCCGCAATAC |
| Internal bar code: | CGAGCTCATAAGGAATTCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 268 |
| LEAP-Seq percent confirming: | 41.0714 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAAGTGAAGTGCCGACGT |
| Suggested primer 2: | CCCACCCATCCAGCATCTTT |