| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.037977 |
| Chromosome: | chromosome 10 |
| Location: | 388991 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g420512 | (1 of 13) 3.1.13.4 - Poly(A)-specific ribonuclease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCGGCGGCGCCGTACCTCCGTACACCAGGCCAACTGCCACCTGGCGG |
| Internal bar code: | GTATAGGTTATACTAGTTGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2243 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATAACCCTGTCCAGCAG |
| Suggested primer 2: | GCAGGAGTAGCAGCGACTAG |