Insertion junction: CLIP2.037989_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.037989
Chromosome:chromosome 8
Location:2938844
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g374700 FAP244 (1 of 2) PTHR14885:SF1 - WD REPEAT-CONTAINING PROTEIN 96; Flagellar Associated Protein 244 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCACACATGCCGTGCTCTCGTGTTATTTCAACGTGGCAATGCAACGACG
Internal bar code:TTGTTTTTCCAGCTCTTTGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:460
LEAP-Seq percent confirming:71.4286
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAAACTTCCAACTGCCGG
Suggested primer 2:CCACACACACACACACACAC