Insertion junction: CLIP2.038046_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.038046
Chromosome:chromosome 3
Location:7668969
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g204050 ELG6 Exostosin-like glycosyltransferase 6; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACACACACACACACACACACACACACACACATGCATCGCATACA
Internal bar code:TGTGTCTCGGGTTGGGGCCTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2251
LEAP-Seq percent confirming:8.47458
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:54
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGAGTCTACGTGTACGGC
Suggested primer 2:GATGGGCTGGTACGGAGATG