| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.038054 |
| Chromosome: | chromosome 10 |
| Location: | 3457670 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444450 | SSA17 | cilia-sensing, structure and/or assembly; (1 of 1) K16462 - centrosomal protein CEP164 (CEP164) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCGCAGCCTTGGAGCCACTGCCCCCGTCCGCCGGTGACCCGCCGAGT |
| Internal bar code: | TTAGAATTGAGGTAACATTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1828 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACATTGAGGAGCTGGTG |
| Suggested primer 2: | CGTTGTCGTGGTCACTCTGA |