| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038081 |
| Chromosome: | chromosome 2 |
| Location: | 5895540 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g117500 | HXK1 | Hexokinase; (1 of 1) K00844 - hexokinase (HK) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTCGACCTCCAGTTCCGCCAACCGCTCCGCGCTGGGGACGAGCTGGTC |
| Internal bar code: | TTTTACGGTTAATTAATAACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1506 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTATCCAACCCATTGCG |
| Suggested primer 2: | CTATGTAGAGCCTGCAGCCC |