Insertion junction: CLIP2.038087_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.038087
Chromosome:chromosome 17
Location:2166417
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g712250 (1 of 4) 2.7.11.13 - Protein kinase C / PKC intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGTCGCAACACAAACTCACCGGCTCCTGTCGGCCTCCCCACACCCGGG
Internal bar code:ACGCTGTCTCGGAAAATACCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:576
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGAGGACCGACCACTTTC
Suggested primer 2:GAAGAGCTGTTGGTCCGTCA