| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.038094 |
| Chromosome: | chromosome 7 |
| Location: | 656388 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317100 | POB18 | Proteome of basal body 18 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTCCAGCATCCCCACTTGCAAGGCACACCACGCACTATCTCCATTTGG |
| Internal bar code: | TTCGTCACCGGTGGGAGGCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3568 |
| LEAP-Seq percent confirming: | 51.9084 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 131 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAGCTCTCGTTCTCGCTG |
| Suggested primer 2: | CTATTCTTTGGGCCTGGGCA |