| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038100 |
| Chromosome: | chromosome 16 |
| Location: | 2981017 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663700 | (1 of 1) K03871 - von Hippel-Lindau disease tumor supressor (VHL) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGAGACCAGGCTTGCCAGCTGCCGGAAGGGCAATGCTGCAGAGCTTAC |
| Internal bar code: | GAGGGGCTAGGAGGCGTACATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1669 |
| LEAP-Seq percent confirming: | 5.40541 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGTAGAATATCCCGCCA |
| Suggested primer 2: | GTGGACCGTTGACACGTTTG |