Insertion junction: CLIP2.038103_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.038103
Chromosome:chromosome 3
Location:1321328
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g150400 PIGU1,PIGU,LRG5 GPI transamidase subunit PIG-U; (1 of 1) K05293 - phosphatidylinositol glycan, class U (PIGU) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTCTGCCACTGTGTCTGGTGCTATTTCGAACCCAATCGATTTCGAGCC
Internal bar code:TTTCAGTTGAAAAGGAGCGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3006
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:TGACAGTTTACAGACGGGCC
Suggested primer 2:AAAACACCACCCCCGAAACT