| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.038107 |
| Chromosome: | chromosome 12 |
| Location: | 3309513 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g498850 | CEP12 | Cysteine endopeptidase; (1 of 3) 3.4.18.1 - Cathepsin X / Lysosomal carboxypeptidase B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGTCCCTTTGCTACCCGGTCGCTCCTCGCCCTCTCATCCCCTCCCCG |
| Internal bar code: | TGTTGCCATACAGAGTTCCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1026 |
| LEAP-Seq percent confirming: | 88.2353 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTAGCCCCAAGAGCTGCTA |
| Suggested primer 2: | CTGCCATCCTTGCAATACGC |