| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.038143 |
| Chromosome: | chromosome 5 |
| Location: | 309997 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g800514 | (1 of 1) PTHR19878//PTHR19878:SF8 - AUTOPHAGY PROTEIN 16-LIKE // AUTOPHAGIC-RELATED PROTEIN 16.1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCATGGCGGATCCGTTTCGCATCGGTTGGAAAGCACGTGTCTAAGCAG |
| Internal bar code: | TTTTGTTGTTGCCTCCCGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 299 |
| LEAP-Seq percent confirming: | 39.6552 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATTGTAAACGCCCCTCCC |
| Suggested primer 2: | GCTAGCAGCTGATGAGGGAG |