Insertion junction: CLIP2.038179_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.038179
Chromosome:chromosome 17
Location:2823957
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g718450 MMP2 Matrix metalloproteinase; (1 of 1) IPR003108 - Growth-arrest-specific protein 2 domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAAGCTCGCAGGCCTGGCGGGACAGGGACGCACACGGAGGCGGCGGCGC
Internal bar code:ATACGGTCATGTCGACGTCCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3491
LEAP-Seq percent confirming:97.9167
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:TTCACAAGTAGTCGGGTGGC
Suggested primer 2:GAAGCACCAACCGTCACAAC