Insertion junction: CLIP2.038183_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.038183
Chromosome:chromosome 6
Location:6278540
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g292500 CYN23b,CYN23 Cyclophilin 23; (1 of 1) PTHR11071//PTHR11071:SF17 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // CYCLOPHILIN-LIKE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FAMILY PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGCCATGCACTGCTAAATCTGTACTGCGCGCTTAAAGCGTTGCGCACT
Internal bar code:TGAGGGTGAGCAGAAATTGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1739
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGGAGGGGTACGAGTTCT
Suggested primer 2:CTCCTTGTCCACCTCCCAAC