| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.038195 |
| Chromosome: | chromosome 10 |
| Location: | 4151232 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448800 | (1 of 1) PF09186 - Domain of unknown function (DUF1949) (DUF1949) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGCCGGGCGGGGGGGGGGGCTGGCGGGGCCCTGGGAACAGGCTGGGT |
| Internal bar code: | AGTGGCATGTTAGAAGCTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 689 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACGCTGTAGAGCCAAAGG |
| Suggested primer 2: | GAGAGCAGGTAGACGATGCC |