Insertion junction: CLIP2.038199_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.038199
Chromosome:chromosome 16
Location:4055563
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g690350 SNR6 SNARE-associated Golgi protein; (1 of 1) PTHR12677:SF24 - TRANSMEMBRANE PROTEIN 64 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCACCATCCGCAGCCCCCATTCACCTCCCCCGCAACTCCACCGCACCTC
Internal bar code:TGCAACTTGTTAAGGAGCTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:197
LEAP-Seq percent confirming:64.7059
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:ACATAGTCTCCGTCCGGTCA
Suggested primer 2:TCGAAGCTCGTTCCCTAGGA