Insertion junction: CLIP2.038225_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.038225
Chromosome:chromosome 17
Location:3438720
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g723500 (1 of 1) 3.6.3.30 - Fe(3+)-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTGCCCTTCCTGACCAGTGTGGGTCTGGCGCCGGCGCTGGACGGCGGC
Internal bar code:AGATGGCGTATCAAATATGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:627
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCTATGTCTACCCCCATGCC
Suggested primer 2:AACGTCCTCATGCTGGTGAG