| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.038228 |
| Chromosome: | chromosome 4 |
| Location: | 3156964 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g226100 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGGAGGAACTCAACGACTCCAACACGTGGTGAGAATTCCTAGCTGTT |
| Internal bar code: | GGTCCAGACTGAAGCGCGAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3200 |
| LEAP-Seq percent confirming: | 95.5224 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCGATATGCTTCACCGA |
| Suggested primer 2: | GCACGCGGATGACAAGAATC |