Insertion junction: CLIP2.038241_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.038241
Chromosome:chromosome 3
Location:6444236
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g193250 (1 of 1) 2.7.1.160 - 2'-phosphotransferase / Yeast 2'-phosphotransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGGAGACTTAGGACCTTTCCCTCCTGGCGATCAGGCCAACATGCCATGC
Internal bar code:GTCCAATGGGCGGGTGACAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:4596
LEAP-Seq percent confirming:99.0099
LEAP-Seq n confirming:100
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:101

Suggested primers for confirmation by PCR

Suggested primer 1:ATCTCAAGAACCAGCCCACG
Suggested primer 2:GCCCACTTTTCCCTTGCAAG