Insertion junction: CLIP2.038243_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.038243
Chromosome:chromosome 6
Location:1827677
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g263650 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCCGACGCGATGCCGTCGATCCCGCTGCTCACGTTGTTGCTGTCGCTG
Internal bar code:GATCGTGATGAGGTCGACTGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1243
LEAP-Seq percent confirming:62.5
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:TATCACGTGCCGCCATAGAC
Suggested primer 2:GGGAAGGGGATGTCTGTGTG