| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.038245 |
| Chromosome: | chromosome 6 |
| Location: | 6329178 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g800716 | (1 of 1) K14015 - nuclear protein localization protein 4 homolog (NPLOC4, NPL4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAACCAAAACATCCAACCCCCCCCCCACCCCACCTGCTGGGTCTTGCTT |
| Internal bar code: | GGCAGCAGCACGTCTGCCTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 187 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGGGCTTCATCTTCAGC |
| Suggested primer 2: | TGGGGTGGGGTTACAGGTTA |