| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.038246 |
| Chromosome: | chromosome 10 |
| Location: | 6453665 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g464900 | (1 of 10) 3.1.1.5 - Lysophospholipase / Phospholipase B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCAGGCCGAGGCTCCTGGTCCCTCTGCTGTCAGGTGCCTGTGTACGGT |
| Internal bar code: | CTGGTCTCGACGCAGGTGTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 94 |
| LEAP-Seq percent confirming: | 7.69231 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACTTGTCCTGAGCGAAC |
| Suggested primer 2: | GATCAGCTGCACTGCAATCG |