| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038250 |
| Chromosome: | chromosome 10 |
| Location: | 3884053 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446300 | SMM36 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 2) PTHR12176//PTHR12176:SF28 - UNCHARACTERIZED // S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE-LIKE PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCACATGTGCCCATGTGCCGATGCGTTCTCCTCCCTCGCCTTCTCAC |
| Internal bar code: | GCGATTTCTTATTCTGTGTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5648 |
| LEAP-Seq percent confirming: | 92.7928 |
| LEAP-Seq n confirming: | 103 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 111 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGCATCACTTAGCTGCT |
| Suggested primer 2: | TGCAGTGCTCGCAGTTTAGA |