Insertion junction: CLIP2.038258_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.038258
Chromosome:chromosome 3
Location:5359178
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g183300 (1 of 4) 2.7.9.5 - Phosphoglucan, water dikinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACATTGCGTTGGTGGCGCTCTGTATCCTCGTGGACACCACAGGTATGTG
Internal bar code:ATTTCTCCCGAGCTATGGCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:776
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CATAGCCAGCGAAAACGACG
Suggested primer 2:CCGCTCATTCTGCATCGTTG