| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.038304 |
| Chromosome: | chromosome 2 |
| Location: | 5305419 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113050 | (1 of 2) PTHR23344:SF28 - GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATATCAGATACGCGGTTACACACTTCCGAACTACGCCAACCTGCGCCT |
| Internal bar code: | GTTGGTCCTTGTAAGTCGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5245 |
| LEAP-Seq percent confirming: | 97.0588 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 102 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGTTGCTCCGCAGAAGA |
| Suggested primer 2: | CGGTTCCATGGCATGCAAAA |